Anti-JUN Polyclonal Antibody (HY261014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HY261014

品牌

abinScience

描述

Anti-JUN Polyclonal Antibody (HY261014) is a rabbit polyclonal antibody detecting JUN in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human JUN (Met1-Phe331).

靶标

Proto-oncogene c-Jun, JUN, V-jun avian sarcoma virus 17 oncogene homolog, Transcription factor AP-1, AP1, Activator protein 1, p39

纯化方式

Purified by antigen affinity column.

Accession号

P05412

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Transcription factor Jun is a ~35 kDa protein. Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity. Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

1. Qing, J. et al. (2000) The Journal of biological chemistry 275, 38802-12. PMID: 10995748

2. Ji, Z. et al. (2012) Molecular and cellular biology 32, 385-98. PMID: 22083952

3. Baumann, S. et al. (2003) Oncogene 22, 1333-9. PMID: 12618758

4. Lan, HC. et al. (2007) Molecular and cellular biology 27, 2027-36. PMID: 17210646

5. Serra, RW. et al. (2014) eLife 3, e02313. PMID: 24623306

Note

For research use only.

图片

参考文献

产品说明书

Anti-JUN Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-JUN Polyclonal Antibody (HY261014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-JUN Polyclonal Antibody (HY261014)

概述

图片

参考文献

产品说明书

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