Anti-YWHAZ Polyclonal Antibody (HX859014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HX859014

品牌

abinScience

描述

Anti-YWHAZ Polyclonal Antibody (HX859014) is a rabbit polyclonal antibody detecting YWHAZ in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human YWHAZ (Met1-Asn245).

靶标

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

纯化方式

Purified by antigen affinity column.

Accession号

P63104

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

14-3-3 protein zeta/delta (YWHAZ) is a ~27 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation.

1. Zheng, W. et al. (2003) Nature structural biology 10, 1054-7. PMID: 14578935

2. Tsuruta, F. et al. (2004) The EMBO journal 23, 1889-99. PMID: 15071501

3. Ganguly, S. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1222-7. PMID: 15644438

4. Gu, YM. et al. (2006) FEBS letters 580, 305-10. PMID: 16376338

5. Angrand, PO. et al. (2006) Molecular & cellular proteomics : MCP 5, 2211-27. PMID: 16959763

6. Popov, IK. et al. (2019) Frontiers in physiology 10, 388. PMID: 31024343

7. Dubois, T. et al. (1997) The Journal of biological chemistry 272, 28882-8. PMID: 9360956

8. Zhang, Z. et al. (2022) Molecular cell 82, 2844-2857.e10. PMID: 35662396

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-YWHAZ polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: Raji cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Hela cell lysate
Lane 8: PC-12 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Mouse kidney lysate
Lane11: Rat brain lysate
Lane12: Rat kidney lysate

参考文献

产品说明书

Anti-YWHAZ Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-YWHAZ Polyclonal Antibody (HX859014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-YWHAZ Polyclonal Antibody (HX859014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价