Anti-Human RTRAF Polyclonal Antibody (HA155014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HA155014

品牌

abinScience

描述

Anti-Human RTRAF Polyclonal Antibody (HA155014) is a rabbit polyclonal antibody detecting RTRAF in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human RTRAF (Phe2-Glu118).

靶标

RNA transcription, translation and transport factor protein, CLE7 homolog, CLE, hCLE, RTRAF, C14orf166, CGI-99

纯化方式

Purified by antigen affinity column.

Accession号

Q9Y224

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

tRNA-splicing ligase complex subunit RTRAF is a ~28 kDa protein. Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Could also play a role in RNA transport.

1. Popow, J. et al. (2011) Science (New York, N.Y.) 331, 760-4. PMID: 21311021

2. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230

3. Pérez-González, A. et al. (2006) Journal of molecular biology 362, 887-900. PMID: 16950395

4. Pérez-González, A. et al. (2014) PloS one 9, e90957. PMID: 24608264

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-RTRAF polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate

参考文献

产品说明书

Anti-Human RTRAF Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human RTRAF Polyclonal Antibody (HA155014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human RTRAF Polyclonal Antibody (HA155014)

概述

图片

参考文献

产品说明书

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