Anti-FUS Polyclonal Antibody (HW826014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HW826014

品牌

abinScience

描述

Anti-FUS Polyclonal Antibody (HW826014) is a rabbit polyclonal antibody detecting FUS in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human FUS (Ser282-Ser367).

靶标

RNA-binding protein FUS, 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein, FUS, TLS

纯化方式

Purified by antigen affinity column.

Accession号

P35637

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

RNA-binding protein FUS is a ~53 kDa protein. DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Also binds its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair.

1. Yamaguchi, A. et al. (2016) Scientific reports 6, 35195. PMID: 27731383

2. Nguyen, CD. et al. (2011) Journal of molecular biology 407, 273-83. PMID: 21256132

3. Yu, Y. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8608-13. PMID: 26124092

4. Zhou, Y. et al. (2013) PLoS genetics 9, e1003895. PMID: 24204307

5. Baechtold, H. et al. (1999) The Journal of biological chemistry 274, 34337-42. PMID: 10567410

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-FUS polyclonal antibody at 1ug/mL on various samples.
Lane 1: N2A cell lysate
Lane 2: HepG2 cell lysate

参考文献

产品说明书

Anti-FUS Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-FUS Polyclonal Antibody (HW826014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-FUS Polyclonal Antibody (HW826014)

概述

图片

参考文献

产品说明书

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