Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014)

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概述
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产品说明书

概述

货号

JN051014

品牌

abinScience

描述

Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014) is a rabbit polyclonal antibody detecting Sortase A in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Staphylococcus aureus

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Staphylococcus aureus srtA/Sortase A (Gln60-Lys206).

靶标

Sortase A, 3.4.22.70, Surface protein sorting A, srtA, SAOUHSC_02834

纯化方式

Purified by antigen affinity column.

Accession号

Q2FV99

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Sortase A (srtA) is a ~23 kDa protein. Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall. This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues. Utilizes lipid II as the peptidoglycan substrate for the sorting reaction.

1. Mazmanian, SK. et al. (1999) Science (New York, N.Y.) 285, 760-3. PMID: 10427003

2. Ton-That, H. et al. (1999) The Journal of biological chemistry 274, 24316-20. PMID: 10446208

3. Ton-That, H. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 12424-9. PMID: 10535938

4. Ton-That, H. et al. (2002) The Journal of biological chemistry 277, 7447-52. PMID: 11714722

5. Kruger, RG. et al. (2004) Biochemistry 43, 1541-51. PMID: 14769030

6. Marraffini, LA. et al. (2004) The Journal of biological chemistry 279, 37763-70. PMID: 15247224

Note

For research use only.

图片

参考文献

产品说明书

Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014)

概述

图片

参考文献

产品说明书

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