Anti-Bacillus cereus gajA Polyclonal Antibody (JN922014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

JN922014

品牌

abinScience

描述

Anti-Bacillus cereus gajA Polyclonal Antibody (JN922014) is a rabbit polyclonal antibody detecting gajA in ELISA, IHC, WB. Suitable for Bacillus cereus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Bacillus cereus

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Bacillus cereus gajA (Gly258-Gly578).

靶标

Endonuclease GajA, 3.1.-.-, Gabija protein GajA, Nicking endonuclease GajA, gajA, IIE_04982

纯化方式

Purified by antigen affinity column.

Accession号

J8H9C1

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Endonuclease GajA is a ~66 kDa protein. Component of antiviral defense system Gabija type I, composed of GajA and GajB. Endonuclease that nicks double-stranded DNA within the sequence 5'-TNNNCGGGNNA-3' in the absence of nucleotides (NTP, dNTP and NDPs), cleaving after C-1. Has no detected ATPase activity. Expression of Gabija type I in B.subtilis (strain BEST7003) confers resistance to phages phi105, phi29, rho14, SpBeta and SBSphiC. Expression of Gabija type I in E.coli B (strain ATCC 11303) confers resistance to phage T7.

1. Doron, S. et al. (2018) Science (New York, N.Y.) 359. PMID: 29371424

2. Cheng, R. et al. (2021) Nucleic acids research 49, 5216-5229. PMID: 33885789

3. Antine, SP. et al. (2024) Nature 625, 360-365. PMID: 37992757

Note

For research use only.

图片

参考文献

产品说明书

Anti-Bacillus cereus gajA Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Bacillus cereus gajA Polyclonal Antibody (JN922014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Bacillus cereus gajA Polyclonal Antibody (JN922014)

概述

图片

参考文献

产品说明书

相关产品推荐

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