Anti-Pseudomonas fluorescens estF/Arylesterase Polyclonal Antibody (JN854014)

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参考文献
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概述

货号

JN854014

品牌

abinScience

描述

Anti-Pseudomonas fluorescens estF/Arylesterase Polyclonal Antibody (JN854014) is a rabbit polyclonal antibody detecting Arylesterase in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Pseudomonas fluorescens

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Pseudomonas fluorescens estF/Arylesterase (Met1-Arg272).

靶标

Arylesterase, 3.1.1.2, Aryl-ester hydrolase, Carboxylic acid perhydrolase, PFE, Putative bromoperoxidase, 1.-.-.-, estF

纯化方式

Purified by antigen affinity column.

Accession号

P22862

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Arylesterase (estF) is a ~30 kDa protein. Hydrolyzes phenolic esters, such as phenyl acetate, nitrophenyl acetate and naphtyl acetate. Can act on a wide range of esters, but reaction rate and enantioselectivity differ significantly depending on the substrate. Shows a preference for esters with small acyl groups. Also shows low perhydrolase activity, and catalyzes the reversible formation of peroxycarboxylic acids from carboxylic acids and hydrogen peroxide. In vitro, enzyme-generated peracetic acid oxidizes bromide ion to bromonium, which reacts with monochlorodimedone to form bromochlorodimedone.

1. Choi, KD. et al. (1990) Agricultural and biological chemistry 54, 2039-45. PMID: 1368608

2. Bernhardt, P. et al. (2005) Angewandte Chemie (International ed. in English) 44, 2742-2746. PMID: 15803517

3. Pelletier, I. et al. (1995) Microbiology (Reading, England) 141 ( Pt 2), 459-68. PMID: 7704276

4. Krebsfänger, N. et al. (1998) Journal of biotechnology 60, 105-11. PMID: 9571805

5. Cheeseman, JD. et al. (2004) Acta crystallographica. Section D, Biological crystallography 60, 1237-43. PMID: 15213385

6. Yin, DL. et al. (2010) Biochemistry 49, 1931-42. PMID: 20112920

7. Yin, DT. et al. (2012) Chemistry (Weinheim an der Bergstrasse, Germany) 18, 8130-9. PMID: 22618813

Note

For research use only.

图片

参考文献

产品说明书

Anti-Pseudomonas fluorescens estF/Arylesterase Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Pseudomonas fluorescens estF/Arylesterase Polyclonal Antibody (JN854014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Pseudomonas fluorescens estF/Arylesterase Polyclonal Antibody (JN854014)

概述

图片

参考文献

产品说明书

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