Anti-Human MPHOSPH10 Polyclonal Antibody (HT215014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HT215014

品牌

abinScience

描述

Anti-Human MPHOSPH10 Polyclonal Antibody (HT215014) is a rabbit polyclonal antibody detecting MPHOSPH10 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human MPHOSPH10 (Asp461-Leu681).

靶标

U3 small nucleolar ribonucleoprotein protein MPP10, M phase phosphoprotein 10, MPHOSPH10, MPP10

纯化方式

Purified by antigen affinity column.

Accession号

O00566

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) is a ~78 kDa protein. Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

1. Granneman, S. et al. (2003) Nucleic acids research 31, 1877-87. PMID: 12655004

2. Singh, S. et al. (2021) Science (New York, N.Y.) 373, eabj5338. PMID: 34516797

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-MPHOSPH10 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate

参考文献

产品说明书

Anti-Human MPHOSPH10 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MPHOSPH10 Polyclonal Antibody (HT215014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MPHOSPH10 Polyclonal Antibody (HT215014)

概述

图片

参考文献

产品说明书

相关产品推荐

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