Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Acyl-coenzyme A thioesterase 8 (ACOT8) is a ~35 kDa protein. Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Displays no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs. Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains. Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT). Is also able to hydrolyze CoA esters of dicarboxylic acids.
1. Ishizuka, M. et al. (2004) Experimental cell research 297, 127-41. PMID: 15194431
2. Liu, LX. et al. (1997) The Journal of biological chemistry 272, 13779-85. PMID: 9153233
3. Watanabe, H. et al. (1997) Biochemical and biophysical research communications 238, 234-9. PMID: 9299485
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-ACOT8 polyclonal antibody at 1ug/mL on various samples. Lane 1: Jurkat cell lysate
