Anti-Human NKRF Polyclonal Antibody (HT276014)

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50ug
100ug

数量：

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概述
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概述

货号

HT276014

品牌

abinScience

描述

Anti-Human NKRF Polyclonal Antibody (HT276014) is a rabbit polyclonal antibody detecting NKRF in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human NKRF (Phe162-Thr415).

靶标

NF-kappa-B-repressing factor, NFkB-repressing factor, NRF, Protein ITBA4, NKRF, ITBA4, NRF

纯化方式

Purified by antigen affinity column.

Accession号

O15226

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

NF-kappa-B-repressing factor (NKRF) is a ~77 kDa protein. Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription. May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes.

1. Feng, X. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 14212-7. PMID: 12381793

2. Nourbakhsh, M. et al. (1999) The EMBO journal 18, 6415-25. PMID: 10562553

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-NKRF polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: K562 cell lysate
Lane 7: A549 cell lysate
Lane 8: Mouse spleen lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat spleen lysate

参考文献

产品说明书

Anti-Human NKRF Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NKRF Polyclonal Antibody (HT276014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NKRF Polyclonal Antibody (HT276014)

概述

图片

参考文献

产品说明书

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