Anti-Human POLR3G Polyclonal Antibody (HT290014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HT290014

品牌

abinScience

描述

Anti-Human POLR3G Polyclonal Antibody (HT290014) is a rabbit polyclonal antibody detecting POLR3G in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human POLR3G (Met1-Met110).

靶标

DNA-directed RNA polymerase III subunit RPC7, RNA polymerase III subunit C7, DNA-directed RNA polymerase III subunit G, RNA polymerase III 32 kDa apha subunit, RPC32-alpha, RNA polymerase III 32 kDa subunit, RPC32, POLR3G

纯化方式

Purified by antigen affinity column.

Accession号

O15318

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA-directed RNA polymerase III subunit RPC7 (POLR3G) is a ~25 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs.

1. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673

2. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764

3. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218

4. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192

5. Haurie, V. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 4176-81. PMID: 20154270

6. Wang, Q. et al. (2021) Nature structural & molecular biology 28, 220-227. PMID: 33558766

7. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254

8. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-POLR3G polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

参考文献

产品说明书

Anti-Human POLR3G Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLR3G Polyclonal Antibody (HT290014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLR3G Polyclonal Antibody (HT290014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价