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Anti-Human RNF13 Polyclonal Antibody (HT135014)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号HT135014
品牌abinScience
描述
Anti-Human RNF13 Polyclonal Antibody (HT135014) is a rabbit polyclonal antibody detecting RNF13 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human RNF13 (Glu33-Glu175).
靶标E3 ubiquitin-protein ligase RNF13, 2.3.2.27, RING finger protein 13, RNF13, RZF
纯化方式Purified by antigen affinity column.
Accession号O43567
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

E3 ubiquitin-protein ligase RNF13 is a ~42 kDa protein. E3 ubiquitin-protein ligase that regulates cell proliferation. Involved in apoptosis regulation. Mediates ER stress-induced activation of JNK signaling pathway and apoptosis by promoting ERN1 activation and splicing of XBP1 mRNA. Also involved in protein trafficking and localization.

1. Zhang, Q. et al. (2009) Cell research 19, 348-57. PMID: 18794910
2. Arshad, M. et al. (2013) The Journal of biological chemistry 288, 8726-8736. PMID: 23378536
3. Edvardson, S. et al. (2019) American journal of human genetics 104, 179-185. PMID: 30595371
4. van Dijk, JR. et al. (2014) The Biochemical journal 459, 27-36. PMID: 24387786
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-RNF13 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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