Anti-Human EIF4E2 Polyclonal Antibody (HT124014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HT124014

品牌

abinScience

描述

Anti-Human EIF4E2 Polyclonal Antibody (HT124014) is a rabbit polyclonal antibody detecting EIF4E2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human EIF4E2 (Glu53-Pro245).

靶标

Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, h4EHP, mRNA cap-binding protein type 3, EIF4E2, EIF4EL3

纯化方式

Purified by antigen affinity column.

Accession号

O60573

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Eukaryotic translation initiation factor 4E type 2 (EIF4E2) is a ~28 kDa protein. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation. In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. In P-bodies, component of a complex that promotes miRNA-mediated translational repression. Involved in virus-induced host response by mediating miRNA MIR34A-induced translational silencing which controls IFNB1 production by a negative feedback mechanism.

1. Rosettani, P. et al. (2007) Journal of molecular biology 368, 691-705. PMID: 17368478

2. Morita, M. et al. (2012) Molecular and cellular biology 32, 3585-93. PMID: 22751931

3. von Stechow, L. et al. (2015) Molecular and cellular biology 35, 1254-68. PMID: 25624349

4. Zhang, X. et al. (2021) Molecular cell 81, 1187-1199.e5. PMID: 33581076

5. Rom, E. et al. (1998) The Journal of biological chemistry 273, 13104-9. PMID: 9582349

6. Chapat, C. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 5425-5430. PMID: 28487484

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human EIF4E2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human EIF4E2 Polyclonal Antibody (HT124014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human EIF4E2 Polyclonal Antibody (HT124014)

概述

图片

参考文献

产品说明书

相关产品推荐

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