Anti-Human MAFK Polyclonal Antibody (HT106014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HT106014

品牌

abinScience

描述

Anti-Human MAFK Polyclonal Antibody (HT106014) is a rabbit polyclonal antibody detecting MAFK in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human MAFK (Met1-Ser156).

靶标

Transcription factor MafK, Erythroid transcription factor NF-E2 p18 subunit, MAFK

纯化方式

Purified by antigen affinity column.

Accession号

O60675

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Transcription factor MafK is a ~17 kDa protein. Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.

1. Toki, T. et al. (1997) Oncogene 14, 1901-10. PMID: 9150357

2. Johnsen, O. et al. (1996) Nucleic acids research 24, 4289-97. PMID: 8932385

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-MAFK polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: A549 cell lysate
Lane 3: Mouse lung lysate

参考文献

产品说明书

Anti-Human MAFK Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MAFK Polyclonal Antibody (HT106014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MAFK Polyclonal Antibody (HT106014)

概述

图片

参考文献

产品说明书

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