Anti-Human TRIM13 Polyclonal Antibody (HF807014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HF807014

品牌

abinScience

描述

Anti-Human TRIM13 Polyclonal Antibody (HF807014) is a rabbit polyclonal antibody detecting TRIM13 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human TRIM13 (Met1-Arg153).

靶标

E3 ubiquitin-protein ligase TRIM13, 2.3.2.27, B-cell chronic lymphocytic leukemia tumor suppressor Leu5, Leukemia-associated protein 5, Putative tumor suppressor RFP2, RING finger protein 77, RING-type E3 ubiquitin transferase TRIM13, Ret finger protein 2, Tripartite motif-containing protein 13, TRIM13, LEU5, RFP2, RNF77

纯化方式

Purified by antigen affinity column.

Accession号

O60858

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

E3 ubiquitin-protein ligase TRIM13 is a ~46 kDa protein. Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Also plays a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway.

1. Tomar, D. et al. (2012) Biochimica et biophysica acta 1823, 316-26. PMID: 22178386

2. Huang, B. et al. (2017) Molecular pharmacology 91, 307-316. PMID: 28087809

3. Hatchi, EM. et al. (2014) The international journal of biochemistry & cell biology 54, 217-22. PMID: 25088585

4. Tomar, D. et al. (2014) Cellular signalling 26, 2606-13. PMID: 25152375

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-TRIM13 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate

参考文献

产品说明书

Anti-Human TRIM13 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRIM13 Polyclonal Antibody (HF807014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRIM13 Polyclonal Antibody (HF807014)

概述

图片

参考文献

产品说明书

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