Anti-Human POLR1A Polyclonal Antibody (HF629014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HF629014

品牌

abinScience

描述

Anti-Human POLR1A Polyclonal Antibody (HF629014) is a rabbit polyclonal antibody detecting POLR1A in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human POLR1A (Asp438-Ala628).

靶标

DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, 2.7.7.6, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194 kDa subunit, RPA194, POLR1A

纯化方式

Purified by antigen affinity column.

Accession号

O95602

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA-directed RNA polymerase I subunit RPA1 (POLR1A) is a ~194 kDa protein. Catalytic core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Transcribes 47S pre-rRNAs from multicopy rRNA gene clusters, giving rise to 5.8S, 18S and 28S ribosomal RNAs. Pol I-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol I pre-initiation complex (PIC) is recruited by the selectivity factor 1 (SL1/TIF-IB) complex bound to the core promoter that precedes an rDNA repeat unit. The PIC assembly bends the promoter favoring the formation of the transcription bubble and promoter escape.

1. Miller, G. et al. (2001) The EMBO journal 20, 1373-82. PMID: 11250903

2. Panov, KI. et al. (2001) Molecular and cellular biology 21, 2641-9. PMID: 11283244

3. Panov, KI. et al. (2006) The EMBO journal 25, 3310-22. PMID: 16858408

4. Zhao, D. et al. (2021) Cell discovery 7, 97. PMID: 34671025

5. Misiaszek, AD. et al. (2021) Nature structural & molecular biology 28, 997-1008. PMID: 34887565

6. Daiß, JL. et al. (2022) Life science alliance 5. PMID: 36271492

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human POLR1A Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLR1A Polyclonal Antibody (HF629014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLR1A Polyclonal Antibody (HF629014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价