Anti-Human ECD Polyclonal Antibody (HF615014)

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概述
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产品说明书

概述

货号

HF615014

品牌

abinScience

描述

Anti-Human ECD Polyclonal Antibody (HF615014) is a rabbit polyclonal antibody detecting ECD in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human ECD (Met10-Asn406).

靶标

Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1, ECD

纯化方式

Purified by antigen affinity column.

Accession号

O95905

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Protein ecdysoneless homolog is a ~72 kDa protein. Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes.

1. Zhang, Y. et al. (2006) Cancer research 66, 7167-75. PMID: 16849563

2. Suh, HW. et al. (2013) Biochemical and biophysical research communications 438, 264-9. PMID: 23880345

3. Kim, JH. et al. (2010) Biological chemistry 391, 9-19. PMID: 19919181

4. Sato, T. et al. (1999) Molecular & general genetics : MGG 260, 535-40. PMID: 9928932

5. Kim, JH. et al. (2009) The Journal of biological chemistry 284, 26402-10. PMID: 19640839

6. Mir, RA. et al. (2015) Molecular and cellular biology 36, 886-99. PMID: 26711270

7. Claudius, AK. et al. (2014) PLoS genetics 10, e1004287. PMID: 24722212

8. Saleem, I. et al. (2021) Molecular and cellular biology 41, e0010321. PMID: 33941617

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ECD polyclonal antibody at ECD on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

参考文献

产品说明书

Anti-Human ECD Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ECD Polyclonal Antibody (HF615014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ECD Polyclonal Antibody (HF615014)

概述

图片

参考文献

产品说明书

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