Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Diphosphoinositol polyphosphate phosphohydrolase 1 (NUDT3) is a ~19 kDa protein. Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. InsP6 (inositol hexakisphosphate) is not a substrate. Acts as a negative regulator of the ERK1/2 pathway. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A) and diadenosine 5',5'''- P1,P5-pentaphosphate (Ap5A) being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A.
1. Yang, X. et al. (1999) The Journal of biological chemistry 274, 35434-40. PMID: 10585413
2. Fisher, DI. et al. (2002) The Journal of biological chemistry 277, 47313-7. PMID: 12370170
3. Safrany, ST. et al. (1998) The EMBO journal 17, 6599-607. PMID: 9822604
4. Safrany, ST. et al. (1999) The Journal of biological chemistry 274, 21735-40. PMID: 10419486
5. Grudzien-Nogalska, E. et al. (2016) RNA (New York, N.Y.) 22, 773-81. PMID: 26932476
6. Sahu, S. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 19245-19253. PMID: 32727897
