Anti-Human CENPB Polyclonal Antibody (HY318014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HY318014

品牌

abinScience

描述

Anti-Human CENPB Polyclonal Antibody (HY318014) is a rabbit polyclonal antibody detecting CENPB in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human CENPB (Ser181-Gly392).

靶标

Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB

纯化方式

Purified by antigen affinity column.

Accession号

P07199

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Major centromere autoantigen B (CENPB) is a ~65 kDa protein. Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable).

1. Tanaka, Y. et al. (2001) The EMBO journal 20, 6612-8. PMID: 11726497

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-CENPB polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: Daudi cell lysate
Lane 7: A549 cell lysate
Lane 8: A431 cell lysate
Lane 9: PC-3 cell lysate

参考文献

产品说明书

Anti-Human CENPB Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CENPB Polyclonal Antibody (HY318014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CENPB Polyclonal Antibody (HY318014)

概述

图片

参考文献

产品说明书

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