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Anti-Human RAP2A Polyclonal Antibody (HY078014)

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概述
货号HY078014
品牌abinScience
描述
Anti-Human RAP2A Polyclonal Antibody (HY078014) is a rabbit polyclonal antibody detecting RAP2A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human RAP2A (Met1-Gln183).
靶标Ras-related protein Rap-2a, 3.6.5.2, RbBP-30, RAP2A
纯化方式Purified by antigen affinity column.
Accession号P10114
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Ras-related protein Rap-2a (RAP2A) is a ~20 kDa protein. Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and regulates cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.

1. Machida, N. et al. (2004) The Journal of biological chemistry 279, 15711-4. PMID: 14966141
2. Taira, K. et al. (2004) The Journal of biological chemistry 279, 49488-96. PMID: 15342639
3. Mittal, V. et al. (2006) The Biochemical journal 394, 309-15. PMID: 16246175
4. Greco, F. et al. (2006) Biochimica et biophysica acta 1763, 330-5. PMID: 16540189
5. Nonaka, H. et al. (2008) Biochemical and biophysical research communications 377, 573-578. PMID: 18930710
6. Kawabe, H. et al. (2010) Neuron 65, 358-72. PMID: 20159449
7. Duncan, ED. et al. (2022) The Journal of cell biology 221. PMID: 35293963
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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