Anti-Human APEH Polyclonal Antibody (HB174014)

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概述
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产品说明书

概述

货号

HB174014

品牌

abinScience

描述

Anti-Human APEH Polyclonal Antibody (HB174014) is a rabbit polyclonal antibody detecting APEH in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human APEH (Ile458-Ser732).

靶标

Acylamino-acid-releasing enzyme, AARE, 3.4.19.1, Acyl-peptide hydrolase, APH, Acylaminoacyl-peptidase, Oxidized protein hydrolase, OPH, APEH, D3F15S2, D3S48E, DNF15S2

纯化方式

Purified by antigen affinity column.

Accession号

P13798

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Acylamino-acid-releasing enzyme (APEH) is a ~81 kDa protein. Aminopeptidase involved in the degradation of N(alpha)-acylated peptides. Displays exopeptidase activity toward N-formyl and N-acetyl peptides with a preference for dipeptide substrates. Catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Hydrolyzes N-formylated peptides likely arising from N-terminal proteolytic cleavage of bacterial and mitochondrial proteins.

1. Fujino, T. et al. (2000) Biochimica et biophysica acta 1478, 102-12. PMID: 10719179

2. Scaloni, A. et al. (1992) The Journal of biological chemistry 267, 3811-8. PMID: 1740429

3. Jones, WM. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 2194-8. PMID: 2006156

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human APEH Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human APEH Polyclonal Antibody (HB174014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human APEH Polyclonal Antibody (HB174014)

概述

图片

参考文献

产品说明书

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