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Anti-Human TUBG1 Polyclonal Antibody (HB833014)

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规格:
  • 50ug
  • 100ug
数量:
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  • 参考文献

  • 产品说明书

概述
货号HB833014
品牌abinScience
描述
Anti-Human TUBG1 Polyclonal Antibody (HB833014) is a rabbit polyclonal antibody detecting TUBG1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human TUBG1 (Met1-Gln451).
靶标Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1, TUBG1, TUBG
纯化方式Purified by antigen affinity column.
Accession号P23258
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Tubulin gamma-1 chain (TUBG1) is a ~51 kDa protein. Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.

1. Brito, C. et al. (2024) Science (New York, N.Y.) 383, 870-876. PMID: 38305685
2. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661
3. Serna, M. et al. (2024) Developmental cell 59, 3175-3188.e8. PMID: 39321809
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-TUBG1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: K562 cell lysate
    Lane 4: Hela cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: A431 cell lysate
    Lane 7: C2C12 cell lysate
    Lane 8: U2OS cell lysate
    Lane 9: A549 cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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