Anti-Human CHKA Polyclonal Antibody (HW469014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HW469014

品牌

abinScience

描述

Anti-Human CHKA Polyclonal Antibody (HW469014) is a rabbit polyclonal antibody detecting CHKA in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human CHKA (Asp138-Leu376).

靶标

Choline kinase alpha, CK, 2.7.1.32, CHETK-alpha, Ethanolamine kinase, EK, 2.7.1.82, CHKA, CHK, CKI

纯化方式

Purified by antigen affinity column.

Accession号

P35790

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Choline kinase alpha (CHKA) is a ~52 kDa protein. Plays a key role in phospholipid biosynthesis by catalyzing the phosphorylation of free choline to phosphocholine, the first step in phosphatidylcholine biosynthesis. Also phosphorylates ethanolamine, thereby contributing to phosphatidylethanolamine biosynthesis. Has higher activity with choline. May contribute to tumor cell growth.

1. Malito, E. et al. (2006) Journal of molecular biology 364, 136-51. PMID: 17007874

2. Gallego-Ortega, D. et al. (2009) PloS one 4, e7819. PMID: 19915674

3. Hudson, CS. et al. (2013) Biochimica et biophysica acta 1834, 1107-16. PMID: 23416529

4. Liu, R. et al. (2021) Molecular cell 81, 2722-2735.e9. PMID: 34077757

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-CHKA polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Raji cell lysate
Lane 3: Mouse testis lysate

参考文献

产品说明书

Anti-Human CHKA Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CHKA Polyclonal Antibody (HW469014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CHKA Polyclonal Antibody (HW469014)

概述

图片

参考文献

产品说明书

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