Anti-Human IgG4 Polyclonal Antibody (HV799014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HV799014

品牌

abinScience

描述

Anti-Human IgG4 Polyclonal Antibody (HV799014) is a rabbit polyclonal antibody detecting Human IgG4 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human IgG4/IGHG4 (Asp95-Asp342).

靶标

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4

纯化方式

Purified by antigen affinity column.

Accession号

P01861

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Immunoglobulin heavy constant gamma 4 (IgG4) is a ~43 kDa protein. Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain.

1. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170

2. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268

3. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-IGHG4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse serum lysate

参考文献

产品说明书

Anti-Human IgG4 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IgG4 Polyclonal Antibody (HV799014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IgG4 Polyclonal Antibody (HV799014)

概述

图片

参考文献

产品说明书

相关产品推荐

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