Anti-Human CRBN Polyclonal Antibody (HP395014)

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概述

货号

HP395014

品牌

abinScience

描述

Anti-Human CRBN Polyclonal Antibody (HP395014) is a rabbit polyclonal antibody detecting CRBN in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human CRBN (Met1-Leu442).

靶标

Protein cereblon, CRBN, AD-006

纯化方式

Purified by antigen affinity column.

Accession号

Q96SW2

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Protein cereblon (CRBN) is a ~50 kDa protein. Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism.

1. Nguyen, TV. et al. (2016) Molecular cell 61, 809-20. PMID: 26990986

2. Lian, Q. et al. (2020) The protein journal 39, 411-421. PMID: 33009960

3. Ito, T. et al. (2010) Science (New York, N.Y.) 327, 1345-50. PMID: 20223979

4. Gandhi, AK. et al. (2014) British journal of haematology 164, 811-21. PMID: 24328678

5. Fischer, ES. et al. (2014) Nature 512, 49-53. PMID: 25043012

6. Chamberlain, PP. et al. (2014) Nature structural & molecular biology 21, 803-9. PMID: 25108355

7. Higgins, JJ. et al. (2008) Neurogenetics 9, 219-23. PMID: 18414909

8. Choi, TY. et al. (2018) The Journal of neuroscience : the official journal of the Society for Neuroscience 38, 3571-3583. PMID: 29530986

9. Kim, MJ. et al. (2019) Frontiers in immunology 10, 2203. PMID: 31620128

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human CRBN Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

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Anti-Human CRBN Polyclonal Antibody (HP395014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CRBN Polyclonal Antibody (HP395014)

概述

图片

参考文献

产品说明书

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