Anti-Human PHF5A Polyclonal Antibody (HA211014)

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50ug
100ug

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概述
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参考文献
产品说明书

概述

货号

HA211014

品牌

abinScience

描述

Anti-Human PHF5A Polyclonal Antibody (HA211014) is a rabbit polyclonal antibody detecting PHF5A in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human PHF5A (Arg21-Arg110).

靶标

PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A

纯化方式

Purified by antigen affinity column.

Accession号

Q7RTV0

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

PHD finger-like domain-containing protein 5A (PHF5A) is a ~12 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, PHF5A is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs.

1. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937

2. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643

3. Teng, T. et al. (2017) Nature communications 8, 15522. PMID: 28541300

4. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

5. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human PHF5A Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PHF5A Polyclonal Antibody (HA211014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PHF5A Polyclonal Antibody (HA211014)

概述

图片

参考文献

产品说明书

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