Anti-Human INTS8 Polyclonal Antibody (HA984014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HA984014

品牌

abinScience

描述

Anti-Human INTS8 Polyclonal Antibody (HA984014) is a rabbit polyclonal antibody detecting INTS8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human INTS8 (Asn831-Phe995).

靶标

Integrator complex subunit 8, Int8, Protein kaonashi-1, C8orf52, INTS8

纯化方式

Purified by antigen affinity column.

Accession号

Q75QN2

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Integrator complex subunit 8 (INTS8) is a ~113 kDa protein. Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Within the integrator complex, INTS8 is required for the recruitment of protein phosphatase 2A (PP2A) to transcription pause-release checkpoint.

1. Oegema, R. et al. (2017) PLoS genetics 13, e1006809. PMID: 28542170

2. Zheng, H. et al. (2020) Science (New York, N.Y.) 370. PMID: 33243860

3. Vervoort, SJ. et al. (2021) Cell 184, 3143-3162.e32. PMID: 34004147

4. Hu, S. et al. (2023) Molecular cell 83, 1588-1604.e5. PMID: 37080207

5. Fianu, I. et al. (2024) Nature 629, 219-227. PMID: 38570683

6. Baillat, D. et al. (2005) Cell 123, 265-76. PMID: 16239144

7. Huang, KL. et al. (2020) Molecular cell 80, 345-358.e9. PMID: 32966759

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human INTS8 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human INTS8 Polyclonal Antibody (HA984014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human INTS8 Polyclonal Antibody (HA984014)

概述

图片

参考文献

产品说明书

相关产品推荐

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