Anti-MACROH2A1 Polyclonal Antibody (HF633014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HF633014

品牌

abinScience

描述

Anti-MACROH2A1 Polyclonal Antibody (HF633014) is a rabbit polyclonal antibody detecting MACROH2A1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human MACROH2A1 (Ile109-Leu369).

靶标

Histone macroH2A1, mH2A1, H2A/y, MACROH2A1, Medulloblastoma antigen MU-MB-50.205, Core histone macro-H2A.1, H2AFY, Histone H2A.y

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

O75367

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Core histone macro-H2A.1 (MACROH2A1) is a ~39 kDa protein. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation.

1. Angelov, D. et al. (2003) Molecular cell 11, 1033-41. PMID: 12718888

2. Zhang, R. et al. (2005) Developmental cell 8, 19-30. PMID: 15621527

3. Doyen, CM. et al. (2006) Molecular and cellular biology 26, 1156-64. PMID: 16428466

4. Hernández-Muñoz, I. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 7635-40. PMID: 15897469

5. Chakravarthy, S. et al. (2005) Molecular and cellular biology 25, 7616-24. PMID: 16107708

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-MACROH1A2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: MCF7 cell lysate
Lane 2: Hela cell lysate
Lane 3: Rat brain lysate
Lane 4: Rat liver lysate
Lane 5: 293T cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse liver lysate
Lane 9: Rat lung lysate

参考文献

产品说明书

Anti-MACROH2A1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MACROH2A1 Polyclonal Antibody (HF633014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MACROH2A1 Polyclonal Antibody (HF633014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价