Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Histone-lysine N-methyltransferase PRDM9 is a ~103 kDa protein. Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4) and 'Lys-36' (H3K36) of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3) and trimethylated 'Lys-36' (H3K36me3) histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Can also methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB) formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression.
1. Miyamoto, T. et al. (2008) Journal of assisted reproduction and genetics 25, 553-7. PMID: 18941885
2. Wu, H. et al. (2013) Cell reports 5, 13-20. PMID: 24095733
3. Eram, MS. et al. (2014) The Journal of biological chemistry 289, 12177-12188. PMID: 24634223
4. Patel, A. et al. (2016) Genes & development 30, 257-65. PMID: 26833727
5. Blazer, LL. et al. (2016) The Journal of biological chemistry 291, 13509-19. PMID: 27129774
