Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Tyrosine-protein phosphatase non-receptor type substrate 1 (CD172a/SIRPA) is a ~56 kDa protein. Immunoglobulin-like cell surface receptor for CD47. Acts as a docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin.
1. Nakaishi, A. et al. (2008) Journal of molecular biology 375, 650-60. PMID: 18045614
2. Sarute, N. et al. (2019) PLoS biology 17, e3000137. PMID: 30726215
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-SIRPA polyclonal antibody at SIRPA on various samples. Lane 1: Mouse brain lysate Lane 2: Mouse cerebellum lysate Lane 3: Rat brain lysate
