Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody (MF562014)

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概述
图片
参考文献
产品说明书

概述

货号

MF562014

品牌

abinScience

描述

Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody (MF562014) is a rabbit polyclonal antibody detecting Adenosine deaminase in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Mouse ADA/Adenosine deaminase (Met1-Gln352).

靶标

Adenosine deaminase, 3.5.4.4, Adenosine aminohydrolase, Ada

纯化方式

Purified by antigen affinity column.

Accession号

P03958

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Adenosine deaminase (ADA) is a ~39 kDa protein. Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.

1. Xu, PA. et al. (2000) Biochemical and biophysical research communications 269, 749-57. PMID: 10720488

2. Mohamedali, KA. et al. (1996) Biochemistry 35, 1672-80. PMID: 8634299

3. Sideraki, V. et al. (1996) Biochemistry 35, 7862-72. PMID: 8672487

4. Sideraki, V. et al. (1996) Biochemistry 35, 15019-28. PMID: 8942668

5. Blackburn, MR. et al. (1997) Development (Cambridge, England) 124, 3089-97. PMID: 9272950

6. Apasov, SG. et al. (2001) The Journal of clinical investigation 108, 131-41. PMID: 11435465

7. Li, G. et al. (2015) Pharmacology research & perspectives 3, e00121. PMID: 26038697

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ADA polyclonal antibody at ADA on various samples.
Lane 1: Jurkat cell lysate
Lane 2: MOLT-4 cell lysate
Lane 3: Mouse thymus lysate
Lane 4: Mouse stomach lysate
Lane 5: Rat spleen lysate

参考文献

产品说明书

Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody (MF562014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody (MF562014)

概述

图片

参考文献

产品说明书

相关产品推荐

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