Anti-Mouse S100A9 Polyclonal Antibody (MY562014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

MY562014

品牌

abinScience

描述

Anti-Mouse S100A9 Polyclonal Antibody (MY562014) is a rabbit polyclonal antibody detecting S100a9 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Mouse S100A9 (Ala2-Lys113).

靶标

Protein S100-A9, Calgranulin-B, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, S100 calcium-binding protein A9, S100a9, Cagb, Mrp14

纯化方式

Purified by antigen affinity column.

Accession号

P31725

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Protein S100-A9 (S100A9) is a ~13 kDa protein. S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Also participates in regulatory T-cell differentiation together with CD69.

1. Vogl, T. et al. (2004) Blood 104, 4260-8. PMID: 15331440

2. Vogl, T. et al. (2007) Nature medicine 13, 1042-9. PMID: 17767165

3. Boyd, JH. et al. (2008) Circulation research 102, 1239-46. PMID: 18403730

4. Björk, P. et al. (2009) PLoS biology 7, e97. PMID: 19402754

5. Riva, M. et al. (2012) Immunology 137, 172-82. PMID: 22804476

6. Daitoku, H. et al. (2021) The Journal of biological chemistry 297, 101230. PMID: 34562450

7. Averill, MM. et al. (2011) Circulation 123, 1216-26. PMID: 21382888

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-S100A9 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse spleen lysate
Lane 2: Rat spleen lysate

参考文献

产品说明书

Anti-Mouse S100A9 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse S100A9 Polyclonal Antibody (MY562014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse S100A9 Polyclonal Antibody (MY562014)

概述

图片

参考文献

产品说明书

相关产品推荐

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