Anti-Mouse GPX1 Polyclonal Antibody (MY365014)

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概述
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参考文献
产品说明书

概述

货号

MY365014

品牌

abinScience

描述

Anti-Mouse GPX1 Polyclonal Antibody (MY365014) is a rabbit polyclonal antibody detecting Gpx1 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Mouse GPX1 (Gly48-Ser201).

靶标

Glutathione peroxidase 1, GPx-1, GSHPx-1, 1.11.1.9, Cellular glutathione peroxidase, Phospholipid-hydroperoxide glutathione peroxidase GPX1, 1.11.1.12, Selenium-dependent glutathione peroxidase 1, Gpx1

纯化方式

Purified by antigen affinity column.

Accession号

P11352

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Glutathione peroxidase 1 (GPX1) is a ~22 kDa protein. Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis. Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides. In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway.

1. Esposito, LA. et al. (2000) Free radical biology & medicine 28, 754-66. PMID: 10754271

2. Wang, SK. et al. (2011) Free radical biology & medicine 51, 197-204. PMID: 21420488

3. Schwarz, M. et al. (2023) Redox biology 59, 102593. PMID: 36608588

4. Esworthy, RS. et al. (1997) Archives of biochemistry and biophysics 340, 59-63. PMID: 9126277

5. Ho, YS. et al. (1997) The Journal of biological chemistry 272, 16644-51. PMID: 9195979

6. de Haan, JB. et al. (1998) The Journal of biological chemistry 273, 22528-36. PMID: 9712879

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-GPX1 polyclonal antibody at GPX1 on various samples.
Lane 1: Mouse liver lysate
Lane 2: Mouse kidney lysate
Lane 3: Mouse spleen lysate
Lane 4: Mouse lung lysate
Lane 5: Rat liver lysate

参考文献

产品说明书

Anti-Mouse GPX1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse GPX1 Polyclonal Antibody (MY365014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse GPX1 Polyclonal Antibody (MY365014)

概述

图片

参考文献

产品说明书

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