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Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody (RB650014)

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  • 100ug
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概述
货号RB650014
品牌abinScience
描述
Anti-Rat CD13/ANPEP/APN Polyclonal Antibody (RB650014) is a rabbit polyclonal antibody detecting ANPEP in ELISA, IHC, WB. Suitable for Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
种属反应性Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Rat CD13/ANPEP/APN (Thr75-Ser280).
经测试应用WB
靶标ANPEP, AP-M, AP-N, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, CD13, EC:3.4.11.2, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150, hAPN
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
纯化方式Purified by antigen affinity column.
Accession号P15684
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Aminopeptidase N (CD13/ANPEP) is a ~108 kDa protein. Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. It is able to degrade Leu-enkephalin and Met-enkephalin but not cholecystokinin CCK8, neuromedin C (GRP-10), somatostatin-14, substance P and vasoactive intestinal peptide.

1. Terashima, H. et al. (1995) Journal of gastroenterology 30, 696-704. PMID: 8963385
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-ANPEP polyclonal antibody at 1ug/mL on various samples.
    Lane 1: PC-3 cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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