Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014)

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概述
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产品说明书

概述

货号

JN045014

品牌

abinScience

描述

Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014) is a rabbit polyclonal antibody detecting cas9 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Streptococcus pyogenes

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Streptococcus pyogenes Cas9 (Met1-Asp1368).

靶标

CRISPR-associated endonuclease Cas9/Csn1, 3.1.-.-, SpCas9, SpyCas9, cas9, csn1, SPy_1046

纯化方式

Purified by antigen affinity column.

Accession号

Q99ZW2

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

CRISPR-associated endonuclease Cas9/Csn1 is a ~158 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing.

1. Deltcheva, E. et al. (2011) Nature 471, 602-7. PMID: 21455174

2. Fonfara, I. et al. (2014) Nucleic acids research 42, 2577-90. PMID: 24270795

3. Sternberg, SH. et al. (2014) Nature 507, 62-7. PMID: 24476820

Note

For research use only.

图片

参考文献

产品说明书

Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014)

概述

图片

参考文献

产品说明书

相关产品推荐

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