Anti-Neisseria meningitidis NadA Polyclonal Antibody (JN971014)

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概述
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参考文献
产品说明书

概述

货号

JN971014

品牌

abinScience

描述

Anti-Neisseria meningitidis NadA Polyclonal Antibody (JN971014) is a rabbit polyclonal antibody detecting autotransporter adhesin NadA in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Neisseria meningitidis

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Neisseria meningitidis NadA (Ala39-Ala170).

靶标

autotransporter adhesin NadA, partial, autotransporter adhesin NadA, partial

纯化方式

Purified by antigen affinity column.

Accession号

P0DV44

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Neisseria adhesin A (NadA) is a ~42 kDa protein. Adheres to and induces bacterial uptake by human epithelial cells in a microfilament-dependent process. Binding is reduced by pronase treatment, suggesting there is a protein receptor on the human cells. Possible human protein receptors include integrin beta-1 (ITGB1) and oxidized low-density lipoprotein receptor 1 (OLR1) (Probable). Binds to extracellular human Hsp90 (preferentially the beta isoform, HSP90AB1) on monocytes, binding stimulates monocytes in a TLR4-dependent fashion, polymixin B, which binds NadA, blocks the activation. Hsp90 is probably not the first receptor on human monocytes.

1. Capecchi, B. et al. (2005) Molecular microbiology 55, 687-98. PMID: 15660996

2. Liguori, A. et al. (2018) mBio 9. PMID: 30327444

3. Cecchini, P. et al. (2011) PloS one 6, e25089. PMID: 21949862

4. Bozza, G. et al. (2014) PloS one 9, e110047. PMID: 25347845

5. Comanducci, M. et al. (2002) The Journal of experimental medicine 195, 1445-54. PMID: 12045242

Note

For research use only.

图片

参考文献

产品说明书

Anti-Neisseria meningitidis NadA Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Neisseria meningitidis NadA Polyclonal Antibody (JN971014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Neisseria meningitidis NadA Polyclonal Antibody (JN971014)

概述

图片

参考文献

产品说明书

相关产品推荐

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