Anti-HAdV-5 E1B-55K Polyclonal Antibody (VK466024)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

VK466024

品牌

abinScience

描述

Anti-HAdV-5 E1B-55K Polyclonal Antibody (VK466024) is a rabbit polyclonal antibody detecting E1B-55K in ELISA, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.

种属反应性

Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)

应用

ELISA, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant HAdV-5 E1B-55K (Glu2-Gln79).

靶标

E1B 55 kDa protein, E1B-55K, E1B protein, large T-antigen, E1B-495R

纯化方式

Purified by antigen affinity column.

Accession号

P03243

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

E1B 55 kDa protein (E1B-55K) is a ~55 kDa protein. Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. E1B-55K probably acts as the substrate-specific adapter of the SCF-like E3 ubiquitin ligase complex. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest.

1. Harada, JN. et al. (2002) Journal of virology 76, 9194-206. PMID: 12186903

2. Carson, CT. et al. (2003) The EMBO journal 22, 6610-20. PMID: 14657032

3. Schwartz, RA. et al. (2008) Journal of virology 82, 9043-55. PMID: 18614635

4. Schreiner, S. et al. (2010) Journal of virology 84, 7029-38. PMID: 20484509

5. Pennella, MA. et al. (2010) Journal of virology 84, 12210-25. PMID: 20861261

6. Wimmer, P. et al. (2016) Oncogene 35, 69-82. PMID: 25772236

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-E1B polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant HAdV-5 E1B-55K/E1B-495R (Catalog No: VK466022)

参考文献

产品说明书

Anti-HAdV-5 E1B-55K Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HAdV-5 E1B-55K Polyclonal Antibody (VK466024)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HAdV-5 E1B-55K Polyclonal Antibody (VK466024)

概述

图片

参考文献

产品说明书

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