Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody (VK734014)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

VK734014

品牌

abinScience

描述

Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody (VK734014) is a rabbit polyclonal antibody detecting Shutoff protein in ELISA, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.

种属反应性

Human adenovirus B serotype 7 (HAdV-7)

应用

ELISA, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant HAdV-7 Shutoff protein/L4 (Leu373-Arg537).

靶标

Shutoff protein, 100 kDa protein, p100K, 100K-chaperone protein, L4-100K, Shutoff protein 100K, L4

纯化方式

Purified by antigen affinity column.

Accession号

Q5EY60

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Shutoff protein (L4) is a ~92 kDa protein. Protein that inhibits host translation while promoting late viral translation by ribosome shunting. Blocks host cap-dependent translation by binding to eIF4G, displacing MKNK1 from cap initiation complexes and preventing EIF4E phosphorylation. Binds to the tripartite leader sequence of viral late mRNAs and recruits host eIF4G, PABPC1/poly-A binding protein and 40S ribosomes subunits on viral mRNAs, allowing ribosome shunting and efficient translation of late viral mRNAs even though conventional translation via ribosome scanning from the cap has been shut off in the host cell. During assembly, acts as a chaperone protein that helps hexon proteins assembly into trimers.

Note

For research use only.

图片

参考文献

产品说明书

Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody (VK734014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody (VK734014)

概述

图片

参考文献

产品说明书

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