Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (VK536014)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

VK536014

品牌

abinScience

描述

Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (VK536014) is a rabbit polyclonal antibody detecting BZLF1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Epstein-Barr virus (HHV-4) (Human herpesvirus 4)

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant EBV/HHV-4 BZLF1 (Leu175-Phe245).

靶标

Lytic switch protein BZLF1, EB1, Protein Z, Trans-activator protein BZLF1, Zta, bZIP transcription factor ZEBRA, BZLF1

纯化方式

Purified by antigen affinity column.

Accession号

P03206

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Lytic switch protein BZLF1 is a ~26 kDa protein. Transcription factor that acts as a molecular switch to induce the transition from the latent to the lytic or productive phase of the virus cycle (Probable). Mediates the switch from the latent to the lytic cycle of infection in cells containing a highly methylated viral genome. Probably binds to silenced chromatin and recruits host chromatin-remodeling enzymes. Regulates this switch by binding to 2 types of ZEBRA response elements (ZREs): the CpG-free AP-1 like elements (latency) and the methylated CpG-containing elements (lytic replication) (Probable). Activates preferentially the methylated forms of the viral lytic R (BRLF1) and Na (BRRF1) gene promoters, the latters being the first genes activated during Z-mediated reactivation in latently infected cells.

1. Schepers, A. et al. (1993) The EMBO journal 12, 3921-9. PMID: 8404860

2. Bhende, PM. et al. (2004) Nature genetics 36, 1099-104. PMID: 15361873

3. Bernaudat, F. et al. (2022) Nucleic acids research 50, 490-511. PMID: 34893887

4. Schaeffner, M. et al. (2019) Life science alliance 2. PMID: 30926617

5. Flower, K. et al. (2011) PloS one 6, e25922. PMID: 22022468

6. Dickerson, SJ. et al. (2009) PLoS pathogens 5, e1000356. PMID: 19325883

7. Feederle, R. et al. (2000) The EMBO journal 19, 3080-9. PMID: 10856251

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-BLZF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EBV/HHV-4 BZLF1 (Catalog No: VK536012)

参考文献

产品说明书

Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (VK536014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (VK536014)

概述

图片

参考文献

产品说明书

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