Anti-Human SAE1 Polyclonal Antibody (HA855014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HA855014

品牌

abinScience

描述

Anti-Human SAE1 Polyclonal Antibody (HA855014) is a rabbit polyclonal antibody detecting SAE1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human SAE1 (Met1-Lys346).

靶标

SAE1, AOS1, Ubiquitin-like 1-activating enzyme E1A, SUA1, SUMO-activating enzyme subunit 1, UBLE1A

纯化方式

Purified by antigen affinity column.

Accession号

Q9UBE0

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

SUMO-activating enzyme subunit 1 (SAE1) is a ~38 kDa protein. The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-SAE1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: HT-29 cell lysate

参考文献

产品说明书

Anti-Human SAE1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SAE1 Polyclonal Antibody (HA855014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SAE1 Polyclonal Antibody (HA855014)

概述

图片

参考文献

产品说明书

相关产品推荐

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