Anti-Human PHF14 Polyclonal Antibody (HF828014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HF828014

品牌

abinScience

描述

Anti-Human PHF14 Polyclonal Antibody (HF828014) is a rabbit polyclonal antibody detecting PHF14 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human PHF14 (Ser296-Arg555).

靶标

PHD finger protein 14, KIAA0783, PHF14

纯化方式

Purified by antigen affinity column.

Accession号

O94880

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

PHD finger protein 14 (PHF14) is a ~106 kDa protein. Histone-binding protein. Binds preferentially to unmodified histone H3 but can also bind to a lesser extent to histone H3 trimethylated at 'Lys-9' (H3K9me3) as well as to histone H3 monomethylated at 'Lys-27' (H3K27ac) and trimethylated at 'Lys-27' (H3K27me3). Represses PDGFRA expression, thus playing a role in regulation of mesenchymal cell proliferation. Suppresses the expression of CDKN1A/p21 by reducing the level of trimethylation of histone H3 'Lys-4', leading to enhanced proliferation of germinal center B cells.

1. Huang, Q. et al. (2013) Acta biochimica et biophysica Sinica 45, 622-33. PMID: 23688586

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-PHF14 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Rat brain lysate

参考文献

产品说明书

Anti-Human PHF14 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PHF14 Polyclonal Antibody (HF828014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PHF14 Polyclonal Antibody (HF828014)

概述

图片

参考文献

产品说明书

相关产品推荐

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