Anti-Human TIGAR Polyclonal Antibody (HA072014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HA072014

品牌

abinScience

描述

Anti-Human TIGAR Polyclonal Antibody (HA072014) is a rabbit polyclonal antibody detecting TIGAR in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human TIGAR (Met1-Arg270).

靶标

Fructose-2, 6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5, TP53-induced glycolysis regulatory phosphatase

纯化方式

Purified by antigen affinity column.

Accession号

Q9NQ88

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Fructose-2,6-bisphosphatase TIGAR is a ~30 kDa protein. Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production. Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death. Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity. In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3.

1. Li, H. et al. (2009) The Journal of biological chemistry 284, 1748-54. PMID: 19015259

2. Bensaad, K. et al. (2006) Cell 126, 107-20. PMID: 16839880

3. Wanka, C. et al. (2012) The Journal of biological chemistry 287, 33436-46. PMID: 22887998

4. Bensaad, K. et al. (2009) The EMBO journal 28, 3015-26. PMID: 19713938

5. Cheung, EC. et al. (2013) Developmental cell 25, 463-77. PMID: 23726973

6. Ye, L. et al. (2013) Biochemical and biophysical research communications 437, 300-6. PMID: 23817040

7. Cheung, EC. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 20491-6. PMID: 23185017

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-TIGAR polyclonal antibody at 1ug/mL on various samples.
Lane 1: Raji cell lysate
Lane 2: HepG2 cell lysate

参考文献

产品说明书

Anti-Human TIGAR Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TIGAR Polyclonal Antibody (HA072014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TIGAR Polyclonal Antibody (HA072014)

概述

图片

参考文献

产品说明书

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