Anti-Human PCMT1 Polyclonal Antibody (HB868014)

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100ug

数量：

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概述
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概述

货号

HB868014

品牌

abinScience

描述

Anti-Human PCMT1 Polyclonal Antibody (HB868014) is a rabbit polyclonal antibody detecting PCMT1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human PCMT1 (Met1-Lys227).

靶标

PIMT, L-isoaspartyl protein carboxyl methyltransferase, PCMT1, Protein-L-isoaspartate(D-aspartate) O-methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase

纯化方式

Purified by antigen affinity column.

Accession号

P22061

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT1) is a ~24 kDa protein. Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.

1. Gilbert, JM. et al. (1988) Biochemistry 27, 5227-33. PMID: 3167043

2. Murray, ED. et al. (1984) The Journal of biological chemistry 259, 10722-32. PMID: 6469980

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-PCMT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K562 cell lysate
Lane 5: Mouse testis lysate
Lane 6: Mouse heart lysate
Lane 7: Rat testis lysate
Lane 8: Rat heart lysate

参考文献

产品说明书

Anti-Human PCMT1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PCMT1 Polyclonal Antibody (HB868014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PCMT1 Polyclonal Antibody (HB868014)

概述

图片

参考文献

产品说明书

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