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Anti-Honeybee Api m 11/MRJP1 Polyclonal Antibody (ZA222014)

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概述
货号ZA222014
品牌abinScience
描述
Anti-Honeybee Api m 11/MRJP1 Polyclonal Antibody (ZA222014) is a rabbit polyclonal antibody detecting MRJP1 in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Apis mellifera (Honeybee)
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Honeybee Api m 11/MRJP1 (Asn20-Leu432).
靶标Major royal jelly protein 1, MRJP-1, MRJP1, 56-kDa protein 4, p56kP-4, Apalbumin 1, Apisin subunit MRJP1, Bee-milk protein, Royalactin, Jellein-1, Jelleine-I, Jellein-2, Jelleine-II, Jellein-4, Jelleine-IV, MRJP1
纯化方式Purified by antigen affinity column.
Accession号O18330
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Major royal jelly protein 1 (Api m 11/MRJP1) is a ~48 kDa protein. Most abundant protein component of royal jelly, a substance produced in the hypopharyngeal gland containing proteins, free amino acids, fatty acids, sugars and other nutrients, which is fed to developing larvae by worker nurse bees. Major royal jelly proteins (Mrjps) are high in essential amino acids and probably have a nutritional function in larval food. All larvae are fed some royal jelly (also known as worker jelly) early in their development but it forms the principal source of nutrition for larvae destined to become queen bees (Probable). Induces the differentiation of honey bee larvae into queens through an Egfr-mediated signaling pathway. Promotes body size increase by activating p70 S6 kinase, stimulates ovary development by augmenting the titer of vitellogenin (Vg) and juvenile hormone, and reduces developmental time by increasing the activity of mitogen-activated protein kinase and inducing 20-hydroxyecdysone (ecdysterone, 20E) production.

1. Santos, KS. et al. (2005) Insect biochemistry and molecular biology 35, 85-91. PMID: 15607658
2. Tamura, S. et al. (2009) Proteomics 9, 5534-43. PMID: 20017154
3. Kamakura, M. (2011) Nature 473, 478-83. PMID: 21516106
4. Dobritzsch, D. et al. (2019) Ecology and evolution 9, 8771-8782. PMID: 31410279
5. Schmitzová, J. et al. (1998) Cellular and molecular life sciences : CMLS 54, 1020-30. PMID: 9791542
6. Buttstedt, A. et al. (2018) Current biology : CB 28, 1095-1100.e3. PMID: 29551410
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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