Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

ZA251014

品牌

abinScience

描述

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014) is a rabbit polyclonal antibody detecting Melittin in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Apis mellifera (Honeybee)

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Honeybee Api m 4/Melittin (Gly44-Gln69).

靶标

Melittin, MEL, MLT, Allergen Api m 3, Allergen Api m III, Api m 4, MELT

纯化方式

Purified by antigen affinity column.

Accession号

P01501

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Melittin (Api m 4) is a ~7 kDa protein. Melittin: Main toxin of bee venom with strong antimicrobial activity and hemolytic activity. It has enhancing effects on bee venom phospholipase A2 activity. This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis. It acts as a voltage-gated pore with higher selectivity for anions over cations. The ion conductance has been shown to be voltage-dependent.

1. Park, D. et al. (2014) Peptides 53, 185-93. PMID: 24512991

2. Tosteson, MT. et al. (1985) The Journal of membrane biology 87, 35-44. PMID: 4057243

3. Schröder, E. et al. (1971) Experientia 27, 764-5. PMID: 5139482

4. Sessa, G. et al. (1969) The Journal of biological chemistry 244, 3575-82. PMID: 5794226

5. Mollay, C. et al. (1974) FEBS letters 46, 141-4. PMID: 4371280

6. Batenburg, AM. et al. (1987) FEBS letters 223, 148-54. PMID: 3666135

7. Hider, RC. et al. (1983) Biochimica et biophysica acta 728, 206-14. PMID: 6830776

8. Tosteson, MT. et al. (1981) Biophysical journal 36, 109-16. PMID: 6269667

9. Kempf, C. et al. (1982) The Journal of biological chemistry 257, 2469-76. PMID: 7061434

Note

For research use only.

图片

参考文献

产品说明书

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

概述

图片

参考文献

产品说明书

相关产品推荐

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