Anti-PEDV Spike glycoprotein S2 Polyclonal Antibody (VK564064)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

VK564064

品牌

abinScience

描述

Anti-PEDV S2/Spike glycoprotein 2 Polyclonal Antibody (VK564064) is a rabbit polyclonal antibody detecting Spike glycoprotein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Porcine epidemic diarrhea virus (PEDV)

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant PEDV S2/Spike glycoprotein 2 (Met961-Gln1386).

经测试应用

靶标

Spike glycoprotein, S glycoprotein, E2, Peplomer protein, S, Porcine epidemic diarrhea virus, PEDV

纯化方式

Purified by antigen affinity column.

Accession号

S0AP48

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Spike glycoprotein (S2/Spike glycoprotein 2) is a ~151 kDa protein. S1 region attaches the virion to the cell membrane by interacting with the host receptor, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-S2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant PEDV S2/Spike glycoprotein 2 (Catalog No: VK564022)

参考文献

产品说明书

Anti-PEDV Spike glycoprotein S2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PEDV Spike glycoprotein S2 Polyclonal Antibody (VK564064)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PEDV Spike glycoprotein S2 Polyclonal Antibody (VK564064)

概述

图片

参考文献

产品说明书

相关产品推荐

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