Anti-Human ULK1 Polyclonal Antibody (HF942014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HF942014

品牌

abinScience

描述

Anti-Human ULK1 Polyclonal Antibody (HF942014) is a rabbit polyclonal antibody detecting ULK1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human ULK1 (Met1-His275).

靶标

ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, KIAA0722, Serine/threonine-protein kinase ULK1, hATG1, ATG1

纯化方式

Purified by antigen affinity column.

Accession号

O75385

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Serine/threonine-protein kinase ULK1 is a ~112 kDa protein. Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences.

1. Chan, EY. et al. (2009) Molecular and cellular biology 29, 157-71. PMID: 18936157

2. Löffler, AS. et al. (2011) Autophagy 7, 696-706. PMID: 21460634

3. Jung, CH. et al. (2011) Autophagy 7, 1212-21. PMID: 21795849

4. Nazio, F. et al. (2013) Nature cell biology 15, 406-16. PMID: 23524951

5. Ro, SH. et al. (2014) The FEBS journal 281, 3816-27. PMID: 25040165

6. Kim, JH. et al. (2018) EMBO reports 19. PMID: 29487085

7. Di Rienzo, M. et al. (2019) Science advances 5, eaau8857. PMID: 31123703

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ULK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate

参考文献

产品说明书

Anti-Human ULK1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ULK1 Polyclonal Antibody (HF942014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ULK1 Polyclonal Antibody (HF942014)

概述

图片

参考文献

产品说明书

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