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Anti-Mouse MMP-9 Recombinant Antibody (SDS3) (MB945013)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

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概述
货号MB945013
品牌abinScience
种属反应性Human, Mouse
应用ELISA, IP
同种型IgG2a, kappa
克隆号SDS3
经测试应用FCM
靶标Matrix metalloproteinase-9, CLG4B, GELB, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, MMP9, MMP-9
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P41245
RRIDAnti-Mouse MMP9 Antibody (SDS3) (abinScience Cat# MB945013, RRID:AB_3725633)
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Matrix metalloproteinase-9 (MMP9) is a ~80 kDa protein. Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments.

1. Reponen, P. et al. (1994) The Journal of cell biology 124, 1091-1102. PMID: 8132709
2. Ahn, BJ. et al. (2012) Biochemical and biophysical research communications 428, 438-44. PMID: 23142597
3. Jeon, S. et al. (2020) Circulation 142, 1736-1751. PMID: 32883094
NoteFor research use only.
Isotype ControlMouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Mouse MMP9 Antibody (SDS3).

  • Flow CytoMetry

    Flow-cytometry using PE anti-human MMP2 antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human MMP2 monoclonal antibody (Catalog: MB945013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using PE anti-human MMP9 antibody. BxPC-3 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human MMP9 monoclonal antibody (Catalog: MB945013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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