Anti-Human IL25/IL17E Recombinant Antibody (2c3) (HV984013)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HV984013

abinScience

种属反应性

Human

ELISA, FCM

IgG1, kappa

Interleukin-25, IL-25, IL17E, IL25, IL-17E, Interleukin-17E

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

Q9H293

Anti-Human IL25/IL17E Antibody (2c3) (abinScience Cat# HV984013, RRID:AB_3725663)

Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Interleukin-25 (IL25/IL17E) is a ~20 kDa protein. Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production. Promotes and augments T-helper type 2 responses locally or systemically. Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction. In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13. Also induces the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways.

1. Wong, CK. et al. (2005) American journal of respiratory cell and molecular biology 33, 186-94. PMID: 15860795

2. Wu, L. et al. (2015) Journal of immunology (Baltimore, Md. : 1950) 194, 4528-34. PMID: 25821217

3. Fort, MM. et al. (2001) Immunity 15, 985-95. PMID: 11754819

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Bioactivity

Detects Human IL25/IL17E in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IL25 antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL25 antibody monoclonal antibody (Catalog # HV984013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human IL25/IL17E Antibody (2c3).

产品说明书

Anti-Human IL25/IL17E Recombinant Antibody (2c3).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IL25/IL17E Recombinant Antibody (2c3) (HV984013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IL25/IL17E Recombinant Antibody (2c3) (HV984013)

概述

图片

参考文献

产品说明书

相关产品推荐

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