Anti-Phospho-POLR2A-CTD (p(YSPTSPSYSPTSPS)) Recombinant Antibody (S7S5) (HB683053)

价格:

规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HB683053

abinScience

种属反应性

Human

IHC, IP, WB

Mouse

Monoclonal

IgG2a, kappa

DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, POLR2A, POLR2, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P24928

Anti-Phospho-POLR2A-CTD (p(YSPTSPSYSPTSPS)) Antibody (S7S5) (abinScience Cat# HB683053, RRID:AB_3725753)

Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

DNA-directed RNA polymerase II subunit RPB1 (p-POLR2A) is a ~217 kDa protein. Catalytic core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol II pre-initiation complex (PIC) is recruited to DNA promoters, with focused-type promoters containing either the initiator (Inr) element, or the TATA-box found in cell-type specific genes and dispersed-type promoters that often contain hypomethylated CpG islands usually found in housekeeping genes. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA.

1. Kassube, SA. et al. (2013) Nature structural & molecular biology 20, 892-9. PMID: 23748380

2. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682

3. Jishage, M. et al. (2018) Nature structural & molecular biology 25, 859-867. PMID: 30190596

4. Kershnar, E. et al. (1998) The Journal of biological chemistry 273, 34444-53. PMID: 9852112

5. Vo Ngoc, L. et al. (2017) Genes & development 31, 6-11. PMID: 28108474

6. Wang, D. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 843-7. PMID: 8381534

8. Yu, Y. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8608-13. PMID: 26124092

For research use only.

SDS-PAGE

SDS-PAGE for Anti-Phospho-POLR2A-CTD (p(YSPTSPSYSPTSPS)) Antibody (S7S5)

产品说明书

Anti-Phospho-POLR2A-CTD (p(YSPTSPSYSPTSPS)) Recombinant Antibody (S7S5).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Phospho-POLR2A-CTD (p(YSPTSPSYSPTSPS)) Recombinant Antibody (S7S5) (HB683053)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Phospho-POLR2A-CTD (p(YSPTSPSYSPTSPS)) Recombinant Antibody (S7S5) (HB683053)

概述

图片

参考文献

产品说明书

相关产品推荐

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